Gaëlle Legube did her undergraduate studies in Life Sciences at the University of Poitiers. She then performed her PhD in Molecular Biology and Genetics at the University Paul Sabatier (Toulouse, France) in the team of Didier Trouche (1999-2003) on histone modifications in human cells. She joined Asifa Akhtar's lab at the EMBL (Germany) for her first post-doc fellowship on dosage compensation in Drosophila (2003-2006). In 2006 she came back to France as a researcher (staff scientist) in the team of Didier Trouche, and became independent as a group leader in 2011.

She developed the DIvA system that relies on the AsiSI restriction enzyme to induce DSBs in human cells (Iacovani et al., 2010).